This section describes how to amplify DNA. For other uses, seelamp"Please refer to the.

LAMP method(Lamppu, abbreviation for Loop-Mediated Isothermal Amplification) is four types of combinations of six regions selected from the sequence of the target gene.PrimerIs a method of amplifying the DNA using a strand displacement reaction.

The primer is designed so that a loop structure is created at the primer binding site of the first amplification product. Since the loop part is single-stranded, the next primer can bind. Special with high strand displacement activityDNA synthaseIs a double strand in the direction of travelDNAWhile dissociating, proceed with its own extension reaction. Finally, an amplification product having a length of about an integral multiple of the original target sequence accumulates in a reaction at 1°C for about 65 hour. Therefore, when the reaction product is electrophoresed, it is ladder-shaped.

PCRCompared with the method, there is no need for denaturation reaction from single strand to double strand, and there is a feature that the reaction proceeds at a constant temperature of 1 to 2°C,Thermal cyclerDoes not need equipment like. Moreover, since the amplification rate is high and the specificity is high (things other than the target are difficult to increase), it is possible to confirm whether or not the template (target) has increased simply by looking at the cloudiness of the reaction solution. Eiken ChemicalHas a trademark right^[1][2].

application

In-vitro diagnostic drug

The following products are Loopamp series for infectious disease testingEiken ChemicalHas been commercialized by.

• Mycobacterium tuberculosisGroup detection reagent kit
• PertussisDetection reagent kit D
• Mycoplasma PDetection reagent kit
• LegionellaDetection reagent kit C
• H1 pdm 2009 influenza virusDetection reagent kit
• Influenza A virusDetection reagent kit
• H5 subtype influenza virusDetection reagent kit
• SARS coronavirusDetection reagent kit
• New Coronavirus 2019 (SARS-CoV-2)Detection reagent kit

Environmental hygiene inspection

• LegionellaDetection reagent kit E (Legionnaires' disease)
• CryptosporidiumDetection reagent kit
• GiardiaDetection reagent kit

footnote

1. ^ “Loop-mediated isothermal amplification of DNA”. Nucleic Acids Res 28 (12): E63. (June 2000). PMID 10871386. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=10871386. 
2. ^ US6410278[1]

References

• Hiroshi Takano, Eiichi Sakai, Taiji Sasaki: “LAMP (Loop-mediated isothermal amplification) principle and application”, Modern Media, Volume 60, No. 7 (2014) [Ayumi of Medical Examination], pp.211-231.

Related item

• PCR method

外部 リンク

• Eiken chemical LAMP method HP
• LAMP primer design support software PrimerExplorer
• Terramex Real-time Turbidimeter